|
| Status |
Public on Sep 09, 2010 |
| Title |
SW5_Cy5 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Patient SW5.01
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
cell type: Lymphoblastoid cell line
disease status: Williams-Beuren syndrome
genome/variation: typical del(7)(q11.23q11.23)
|
| Growth protocol |
LCLs were grown in RPMI 1640 medium (GIBCO) supplemented with 10% heat-inactivated fetal bovine serum and 1X antibiotics (penicillin/streptomycin) (Invitrogen), at 37ºC in a humidified 5% CO2 chamber.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy5
|
| Label protocol |
Total RNA (500 ngr) from patients and controls was labeled using the Low Input linear RNA amplification kit and purified with RNeasy (Qiagen) following manufacturer's instructions.
|
| |
|
| Channel 2 |
| Source name |
Pool of 5 control cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: Lymphoblastoid cell line
|
| Growth protocol |
LCLs were grown in RPMI 1640 medium (GIBCO) supplemented with 10% heat-inactivated fetal bovine serum and 1X antibiotics (penicillin/streptomycin) (Invitrogen), at 37ºC in a humidified 5% CO2 chamber.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (500 ngr) from patients and controls was labeled using the Low Input linear RNA amplification kit and purified with RNeasy (Qiagen) following manufacturer's instructions.
|
| |
|
| |
| Hybridization protocol |
Hybridization process was performed with the Hybridization Buffer from Agilent for 18h at 60ºC in a rotator oven.
|
| Scan protocol |
Image analysis was performed using the Agilent scaner G2565BA.
|
| Description |
MO__251239139163__SW501_Cy5_pool_Cy3.gpr
|
| Data processing |
The normalization procedure involves loess without background correction.
|
| |
|
| Submission date |
Sep 21, 2009 |
| Last update date |
Sep 09, 2010 |
| Contact name |
Anna Antonell |
| E-mail(s) |
antonell@clinic.ub.es
|
| Organization name |
Hospital Clínic Barcelona
|
| Department |
Unitat d'Alzheimer i altres transtorns cognitius
|
| Lab |
Malalties neurodegeneratives
|
| Street address |
C/Casanova, 143
|
| City |
Barcelona |
| ZIP/Postal code |
08036 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE18188 |
Williams-Beuren syndrome: typical deletions versus atypical deletions of the 7q11.23 region |
|
