|
| Status |
Public on Mar 26, 2007 |
| Title |
Diseased 6 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
motor cortex, reference
|
| Organism |
Homo sapiens |
| Characteristics |
Sample ID: Diseased 2; Sample Type: tissue; Order Code: BTB3292 [Brain and Tissue Bank for Developmental Disorders (Miami, FL, USA)]; Chief Disease: ALS; Cause of Death: ALS; Age [years / days]: 76 / 197; Gender: Male; Race: Caucasian; PMI [h]: 24.
|
| Extracted molecule |
total RNA |
| Label |
Cy3
|
| |
|
| Channel 2 |
| Source name |
motor cortex, diseased 6
|
| Organism |
Homo sapiens |
| Characteristics |
Sample ID: Diseased 6; Sample Type: tissue; Order Code: BTB3590 [Brain and Tissue Bank for Developmental Disorders (Miami, FL, USA)]; Chief Disease: ALS; Cause of Death: pneumonia; Age [years / days]: 75 / -; Gender: Male; Race: Caucasian; PMI [h]: 24.
|
| Extracted molecule |
total RNA |
| Label |
Cy5
|
| |
|
| |
| Description |
Complementary RNAs (cRNAs) labelled with Cy5-CTP (Perkin-Elmer) were synthesized from 1 µg of total RNA of each sample using the Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) following the manufacturer’s protocol. A reference cRNA, labelled with Cy3-CTP (Perkin-Elmer), was synthesized from 1 µg of sample Diseased 2 RNA. Aliquots (750 ng) of Cy3 and Cy5 labelled cRNA targets were co-hybridized on Whole Human Genome Oligo Microarrays (Agilent Technologies). Microarray hybridization and washing were performed using reagents and instruments (hybridization chambers and rotating oven) as indicated by the manufacturer (Agilent Technologies). Microarrays were scanned at 10-μm resolution using a GenePix Personal 4100A microarray scanner and the GenePix Pro 6.0 acquisition and data-extraction software (Molecular Devices, Corp.).
|
| Data processing |
No Normalization
|
| |
|
| Submission date |
Apr 03, 2006 |
| Last update date |
Mar 26, 2007 |
| Contact name |
Sebastiano Cavallaro |
| E-mail(s) |
s.cavallaro@isn.cnr.it
|
| Phone |
+3909577262411
|
| Fax |
+390957122426
|
| URL |
http://web.tiscali.it/sebastiano_cavallaro/
|
| Organization name |
Italian National Research Council
|
| Department |
Institute of Neurological Sciences
|
| Lab |
Functional Genomics Center
|
| Street address |
Viale Regina Margherita, 6
|
| City |
Catania |
| ZIP/Postal code |
95123 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1708 |
| Series (1) |
| GSE4595 |
Expression profiling of motor cortex in sporadic amyotrophic lateral sclerosis |
|
