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Status |
Public on Nov 01, 2007 |
Title |
Novel mechanisms of galactose toxicity in human galactose-1-phosphate uridyltransferase (GALT)-deficient cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
Comparison of gene expression profiles in GALT-deficient and GALT-reconstituted cells showed that cells lacking GALT activity responded to galactose challenge by activating a set of genes characteristic of endoplasmic reticulum (ER) stress. This response was specific to galactose insult, as cells grown in glucose or hexose-free media did not exhibit ER stress. Keywords: GALT infected cells
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Overall design |
Cells were first grown and then infected in high glucose, then transferred to DMEM, 3 experiments with dye-swap
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Contributor(s) |
Lai K, Slepak TI, Slepak VZ |
Citation missing |
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Submission date |
May 18, 2006 |
Last update date |
Dec 06, 2012 |
Contact name |
Kent Lai |
E-mail(s) |
klai@med.miami.edu
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Organization name |
University of Miami
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Department |
Pediatrics
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Street address |
P.O. Box 016820
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City |
Miami |
State/province |
FL |
ZIP/Postal code |
33101 |
Country |
USA |
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Platforms (1) |
GPL1708 |
Agilent-012391 Whole Human Genome Oligo Microarray G4112A (Feature Number version) |
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Samples (6)
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GSM109331 |
Q188Rfibroblasts_glucose_2days_rep1 |
GSM109332 |
Q188Rfibroblasts_glucose_2days_rep2 |
GSM109333 |
Q188Rfibroblasts_hexosefree_2days_rep2 |
GSM109334 |
Q188Rfibroblasts_hexosefree_2days_rep1 |
GSM109335 |
Q188Rfibroblasts_galactose_2days_rep1 |
GSM109336 |
Q188Rfibroblasts_galactose_2days_rep2 |
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Relations |
BioProject |
PRJNA95751 |