|
| Status |
Public on Sep 30, 2012 |
| Title |
Cell line 5 |
| Sample type |
RNA |
| |
|
| Source name |
oral squamous cell carcinoma cell lines
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KB
cell type: oral squamous cell carcinoma
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After fresh tissues were homogenized by TissueLyser, total RNA was extracted by ISOGEN.
|
| Label |
digoxigenin
|
| Label protocol |
Digoxigenin-UTP-labelled cRNA was generated and amplified from 1 mg of total RNA using Applied Biosystems Chemiluminescent RT-IVT Labelling Kit.
|
| |
|
| Hybridization protocol |
Ten mg of labelled cRNA was hybridized to each pre-hybed microarray at 55℃ for 16 hours.
|
| Scan protocol |
After Chemiluminescence detection using Applied Biosystems Chemiluminescence Detection Kit, images were collected by the AB1700 Chemiluminescent Microarray Aanalyzer.
|
| Description |
KB
|
| Data processing |
The robust multichip average (RMA) method was used background correction and normalization.
|
| |
|
| Submission date |
Feb 27, 2012 |
| Last update date |
Sep 30, 2012 |
| Contact name |
Koh-ichi Nakashiro |
| E-mail(s) |
nakako@m.ehime-u.ac.jp
|
| Phone |
+81899605393
|
| Organization name |
Ehime University Graduate School of Medicine
|
| Department |
Oral and Maxillofacial Surgery
|
| Street address |
454 Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1426 |
| Series (1) |
| GSE36090 |
Gene expression profiles in human oral squamous cell carcinoma tissues and cell lines |
|
