|
| Status |
Public on Jan 10, 2011 |
| Title |
LN229_KO_neg_r2 |
| Sample type |
RNA |
| |
|
| Source name |
mock knockdown
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LN229 glioma cancer cells
|
| Treatment protocol |
Sox2 was knockdown in sw620 cell lines by SiRNA and mock no target conrtol SiRNA
|
| Growth protocol |
Cells were cultured in DMEM cell culture media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
start with 250ng total RNA, and Biotinylated cRNA were prepared according to the standard (Applied Biosystems protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 2.0 ug of cRNA were hybridized to hybridized to a 60-mer whole-genome oligonucleotide microarray for 16 hr at 45C and were washed and stained.
|
| Scan protocol |
The chips were scanned with 1700 instrument to detect and measure the chemiluminescent and fluorescent signals.
|
| Description |
mock knockdown LN229 cells
|
| Data processing |
ABI raw intensities were imported into GeneSpring for normalization and data analysis. The ABI Column S_N (signal to noise ratios) was used to filter data. GeneSpring 10.0, is used for automatic normalizing the chip data and analysis the fold change.
|
| |
|
| Submission date |
Aug 26, 2010 |
| Last update date |
Jan 10, 2011 |
| Contact name |
Biaoyang Lin |
| Organization name |
ZheJiang University
|
| Department |
ZCNI
|
| Lab |
Systems Biology Division
|
| Street address |
866 Yu Hang Tang Road Zi Jin Gang Campus Zhejiang University.
|
| City |
HangZhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL1426 |
| Series (2) |
| GSE23838 |
Genes regulated by Sox2 in glioma cancer cell line |
| GSE23839 |
An integrative analysis of the SOX2 response program in glioblastoma |
|
