|
| Status |
Public on Dec 22, 2008 |
| Title |
Endometriosis-16 |
| Sample type |
RNA |
| |
|
| Source name |
endometriosis, broard ligament
|
| Organism |
Homo sapiens |
| Characteristics |
endometriosis, broard ligament
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA and Qiagen RNeasy purification were performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA was hybridized for 16 hr at 45C on GeneChipHuman U133A arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip 2500 Scanner (Affymetrix).
|
| Description |
16BL
|
| Data processing |
MAS5.0
|
| |
|
| Submission date |
Jun 05, 2008 |
| Last update date |
Dec 22, 2008 |
| Contact name |
D. Stephen Charnock-Jones |
| E-mail(s) |
dscj1@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Obstetrics and Gynaecology
|
| Street address |
The Rosie Hospital
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0SW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE11691 |
Euctopic and ectopic human endometrium (endometriosis) |
| GSE11769 |
Analysis of ectopic human endometrium and peritoneal tissues in nude mice |
|
