|
| Status |
Public on Aug 23, 2006 |
| Title |
PatientDD Pretreatment RNA_rep1 (DD109Pre) |
| Sample type |
RNA |
| |
|
| Source name |
PatientDD Pretreatment RNA_rep1
|
| Organism |
Homo sapiens |
| Characteristics |
PBMC
|
| Biomaterial provider |
Donor Patient one day before IFN(Beta)1a (AVONEX, Biogen-Idec) therapy initiation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PBMC total RNA is prepared by RNAeasy protocol. RNA quality checked on bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
The Codelink Reagents for IVT kit is used to label 2 µg of total RNA to produce UTP-biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
Standard CodeLink procedures
|
| Scan protocol |
Standard CodeLink procedures
|
| Description |
Bitynylated cRNA is probe is prepared and hybridization is carried out following the Codelink protocols and reagents
|
| Data processing |
Normalized intensities analysed by by SAM using two fold cutoff.
|
| |
|
| Submission date |
Aug 21, 2006 |
| Last update date |
Aug 22, 2006 |
| Contact name |
Mandal. K. Singh |
| E-mail(s) |
mandal.singh@chp.edu
|
| Phone |
412-692-8402
|
| Fax |
412-692-6918
|
| Organization name |
Childrens Hospital of Pittsburgh
|
| Department |
Transplant
|
| Street address |
3460 Fifth Ave
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15206 |
| Country |
USA |
| |
|
| Platform ID |
GPL4191 |
| Series (1) |
| GSE5574 |
Gene expression in PBMC from Multiple Sclerosis patients undergoing interferon beta therapy. |
|
