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| Status |
Public on Jul 07, 2006 |
| Title |
GAS41 over-expression in Saos2 Tet-Off cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcription initiation in eukaryotes by RNA polymerase II requires numerous general and regulatory factors including the general transcription factors. Here, we report a new cofactor of the general transcription factor IIF, GAS41, which was previously implicated in tumor development. Microarray analysis of cells with induced overexpression of cofactor GAS41 identifies a significant number of up-regulated transcripts (p-value of 0.01).
Keywords: altered gene expression
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| Overall design |
Saos2 Tet-Off clone B3-Dox, with induced GAS41 overexpression, was compared to Saos2 Tet-Off clone B3+Dox, without overexpression. Additionally, B3-Dox was compared to Saos2 Tet-Off clones F7 +Dox/ -Dox, both transfected with pTRE2pur response plasmid only, to exclude any doxycycline effect. All experiments were done in triplicates, revealing twelve data sets.
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| |
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| Contributor(s) |
Heisel S, Schuetz N |
| Citation missing |
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| Submission date |
Jul 03, 2006 |
| Last update date |
Oct 28, 2014 |
| Contact name |
Sabrina M. Heisel |
| E-mail(s) |
s.heisel@mx.uni-saarland.de
|
| Phone |
06841-1626189
|
| Fax |
06841-1626185
|
| Organization name |
Saarland University
|
| Department |
Department of Human Genetics
|
| Street address |
Kirrbergerstrasse
|
| City |
Homburg/Saar |
| ZIP/Postal code |
66421 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL2895 |
GE Healthcare/Amersham Biosciences CodeLink Human Whole Genome Bioarray |
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| Samples (12)
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| GSM118258 |
Saos2 Tet-Off clone F7_control +DOX_rep1 |
| GSM118259 |
Saos2 Tet-Off clone F7_control -DOX_rep1 |
| GSM118260 |
Saos2 Tet-Off clone B3+DOX_rep1 |
| GSM118261 |
Saos2 Tet-Off clone B3-DOX_rep1 |
| GSM118262 |
Saos2 Tet-Off clone F7_control +DOX_rep2 |
| GSM118263 |
Saos2 Tet-Off clone F7_control -DOX_rep2 |
| GSM118264 |
Saos2 Tet-Off clone B3+DOX_rep2 |
| GSM118265 |
Saos2 Tet-Off clone B3-DOX_rep2 |
| GSM118266 |
Saos2 Tet-Off clone F7_control +DOX_rep3 |
| GSM118267 |
Saos2 Tet-Off clone F7_control -DOX_rep3 |
| GSM118268 |
Saos2 Tet-Off clone B3+DOX_rep3 |
| GSM118269 |
Saos2 Tet-Off clone B3-DOX_rep3 |
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| Relations |
| BioProject |
PRJNA96511 |
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