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Series GSE344 Query DataSets for GSE344
Status Public on Mar 18, 2003
Title Spotted long oligonucleotide arrays
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Both spotted long oligonucleotide arrays and Affymetrix GeneChips were used to measure differential gene expression in two RNA samples (K562 erythroleukemia RNA and the Stratagene Universal Human Reference RNA).

For Affymetrix technology, the two RNAs were analyzed separately. There are two replicates for K562 RNA (GSM4843 and GSM4844) and three for the Stratagene Universal reference (GSM4845-GSM4847).

Two types of spotted long oligonucleotide arrays were used. These arrays were used to do two-color hybridizations to directly compare K562 and Universal reference RNAs.

One array (GPL273) was made with probes from the Operon Human Genome Oligo Set Version 1. These arrays were used with unamplified cDNA probes (6 replicates, GSM4848-GSM4853), with aRNA probes produced by one round of T7 RNA polymerase-based amplification (6 replicates, GSM4854-GSM4859), and with aRNA probes produced by two rounds of amplification (2 replicates, GSM4860-GSM4861).
Keywords: parallel sample
 
 
Contributor(s) Barczak A, Rodriguez MW, Hanspers K, Koth LL, Tai YC, Bolstad BM, Speed TP, Erle DJ
Citation(s) 12805270
Submission date Mar 17, 2003
Last update date Dec 13, 2018
Contact name David J Erle
E-mail(s) erle@itsa.ucsf.edu
Phone 415-206-5905
URL http://arrays.ucsf.edu
Organization name University of California San Francisco
Department Medicine
Lab Shared Microarray Facility
Street address Box 0854
City San Francisco
State/province CA
ZIP/Postal code 94143-0854
Country USA
 
Platforms (3)
GPL273 UCSF 3OPHs version 1 human long oligo array
GPL274 UCSF 4Hs version 2 human long oligo array
GPL8300 [HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array
Samples (23)
GSM4843 K562 GeneChip 1
GSM4844 K562 GeneChip 2
GSM4845 Universal reference GeneChip 1
Relations
BioProject PRJNA84827

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE344_RAW.tar 13.4 Mb (http)(custom) TAR (of CEL)

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