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Status
Public on May 17, 2006
Title
NCI/ATC Hs-OperonV3
Technology type
spotted oligonucleotide
Distribution
non-commercial
Organism
Homo sapiens Manufacturer
Advanced Technology Center Microarray Facility (NIH/NCI/CCR/ATC)
Manufacture protocol
Microarrays are printed with GeneMachines robotic arrayer from Genomic Solutions (http://www.genomicsolutions.com ) that is fitted with a printhead containing 48 SMP2.5 printing pins from Telechem International (http://www.arrayit.com ). The human long oligonuleotide probe set contains 34,960 optimized 70mers (plus controls) obtained from Operon (http://www.operon.com/ ). The long oligonucleotide probes were purchased in 384-well polypropylene plates (Genetix, 384 microarray) and dissolved print buffer of sodium phosphate (150mM) at ph 8.5 and 4% sodium sulphate at a concentration of 20-30 uM. The microarray spots are printed with 160 micron column x 155 microns row spacing, and one spot is printed per probe per slide. The microarrays were printed on Corning Expoxide slides or Schott Epoxide slides and post-processed according to the manufacturer's protocol.
Support
glass
Coating
epoxide
Submission date
May 17, 2006
Last update date
May 17, 2006
Contact name
David Petersen
E-mail(s)
petersed@mail.nih.gov Phone
301-435-7898
Organization name
NCI
Lab
NCI Microarray Facility
Street address
8717 Grovemont Circle
City
Gaithersburg
State/province
MD
ZIP/Postal code
20892-4605
Country
USA
Samples (76)
GSM110526 , GSM110527 , GSM110528 , GSM110529 , GSM110530 , GSM110531
GSM272412 ,
GSM272413 ,
GSM272414 ,
GSM272415 ,
GSM272416 ,
GSM272417 ,
GSM343494 ,
GSM343495 ,
GSM343496 ,
GSM343497 ,
GSM547580 ,
GSM547581 ,
GSM547582 ,
GSM547583 ,
GSM547584 ,
GSM547585 ,
GSM547586 ,
GSM547587 ,
GSM547588 ,
GSM547589 ,
GSM547590 ,
GSM547591 ,
GSM547592 ,
GSM547593 ,
GSM547594 ,
GSM765435 ,
GSM765436 ,
GSM765437 ,
GSM765438 ,
GSM765439 ,
GSM765440 ,
GSM765441 ,
GSM765442 ,
GSM765443 ,
GSM765444 ,
GSM765445 ,
GSM765446 ,
GSM765447 ,
GSM765448 ,
GSM765449 ,
GSM765450 ,
GSM765451 ,
GSM765452 ,
GSM765453 ,
GSM765454 ,
GSM765455 ,
GSM765456 ,
GSM765457 ,
GSM765458 ,
GSM765459 ,
GSM765460 ,
GSM765461 ,
GSM765462 ,
GSM765463 ,
GSM765464 ,
GSM765465 ,
GSM765466 ,
GSM765467 ,
GSM765468 ,
GSM765469 ,
GSM765470 ,
GSM765471 ,
GSM765472 ,
GSM765473 ,
GSM765474 ,
GSM765475 ,
GSM765476 ,
GSM765477 ,
GSM765478 ,
GSM765479 Series (5)
GSE4914 RNAi analysis of the FBP transactivator family
GSE10789 Induction of the IL-9 gene by HTLV-I Tax stimulates the spontaneous proliferation of primary adult T-cell leukemia cells
GSE13646 Global effect of JTV or JTV-Alt overexpression in HeLa cells
GSE22022 Microarray profiling of expression patterns of fibroblasts from different skin types
GSE30849 Type I Interferon Dependent Anti-viral Activity
Data table header descriptions ID NIH mAdb well id plus replicate number
MADB_WELL_ID NIH mAdb plate-well identifier for oligo sample
OLIGO_ID Oligo identifier
GENE Gene Name
UNIGENE UniGene Identifier Build #Hs.190
DESCRIPTION Gene Description
GB_ACC GENBANK ID assigned by vendor
REFSEQ_Acc RefSeq ID assigned by mAdb
SPOT_ID spot identifier
Data table ID MADB_WELL_ID OLIGO_ID GENE UNIGENE DESCRIPTION GB_ACC REFSEQ_Acc SPOT_ID 1727120_1
1727120
H200000226
FKBP4
524183
FK506 binding protein 4, 59kDa (FKBP4), mRNA.
NM_002014 NM_002014
1727132_1
1727132
H300015533
CAMKK1
8417
calcium/calmodulin-dependent protein kinase kinase 1, alpha (CAMKK1), transcript variant 3, mRNA.
NM_172207 NM_172207
1727216_1
1727216
H300016407
BLU PROTEIN
BLU PROTEIN
1727228_1
1727228
H300013738
SUPPRESSION OF TUMORIGENICITY 7, ISOFORM A; HELG PROTEIN; SUPPRESSION OF TUMORIGENICITY 7 (BREAST); FAMILY WITH SEQUENCE SIMILARITY 4, SUBFAMILY A, MEMBER 1
SUPPRESSION OF TUMORIGENICITY 7, ISOFORM A; HELG PROTEIN; SUPPRESSION OF TUMORIGENICITY 7 (BREAST); FAMILY WITH SEQUENCE SIMILARITY 4, SUBFAMILY A, MEMBER 1
1727312_1
1727312
H200016156
HOXA11
249171
homeobox A11 (HOXA11), mRNA.
NM_005523 NM_005523
1727324_1
1727324
H300010851
MEOX1
mesenchyme homeobox 1 (MEOX1), transcript variant 3, mRNA.
NM_004527 NM_001040002
1727408_1
1727408
H200010569
NF1
567266
neurofibromin 1 (neurofibromatosis, von Recklinghausen disease, Watson disease) (NF1), mRNA.
NM_000267 NM_000267
1727420_1
1727420
H300018387
BZRAP1
112499
benzodiazapine receptor (peripheral) associated protein 1 (BZRAP1), mRNA.
NM_004758 NM_004758
1727504_1
1727504
H200017528
RANBP9
587552
RAN binding protein 9 (RANBP9), mRNA.
NM_005493 NM_005493
1727516_1
1727516
H200002827
KIAA0859
494705
KIAA0859 (KIAA0859), transcript variant 3, mRNA.
NM_015935 NM_001007239
1727600_1
1727600
H200002595
NT5M
513977
5',3'-nucleotidase, mitochondrial (NT5M), nuclear gene encoding mitochondrial protein, mRNA.
NM_020201 NM_020201
1727612_1
1727612
H200019995
NISCHARIN; IMIDAZOLINE RECEPTOR CANDIDATE
NISCHARIN; IMIDAZOLINE RECEPTOR CANDIDATE
1727696_1
1727696
H300011466
BTK
159494
Bruton agammaglobulinemia tyrosine kinase (BTK), mRNA.
NM_000061 NM_000061
1727708_1
1727708
H300022292
HFE
233325
hemochromatosis (HFE), transcript variant 5, mRNA.
NM_139005 NM_139005
1727792_1
1727792
H200012859
TSPAN9
504517
tetraspanin 9 (TSPAN9), mRNA.
NM_006675 NM_006675
1727804_1
1727804
H300012994
MKS1
408843
Meckel syndrome, type 1 (MKS1), mRNA.
NM_017777 NM_017777
1727888_1
1727888
H300011928
Unknown
Unknown
1727900_1
1727900
H200004982
RB1CC1
196102
RB1-inducible coiled-coil 1 (RB1CC1), mRNA.
NM_014781 NM_014781
1727984_1
1727984
H300002390
ACCN5
381349
amiloride-sensitive cation channel 5, intestinal (ACCN5), mRNA.
AJ252011 NM_017419
1727996_1
1727996
H200002468
UBR2
529925
Ubiquitin protein ligase E3 component n-recognin 2
NM_015255
35024 3846 Kbytes .Supplementary data files not provided
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