GEO DataSets

Analysis of RB1-depleted primary osteoblasts exposed to 4 Gy of ionizing radiation (IR) for up to 24 hours. IR exposure and germline mutations in RB1 are risk factors for developing osteosarcoma. Results provide insight into the role of RB1 in the response of osteoblasts to IR.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 protocol, 6 time sets

Platform:

GPL6244

Series:

GSE50532

36 Samples

Download data: CEL

(Submitter supplied) The role of RB1 in response to radiation was examined in human osteoblasts. We demonstrate that RB1 induced SASP genes, a response which was attenuated in RB1 knockdown osteoblasts.

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS5350

Platform:

GPL6244

36 Samples

Download data: CEL

(Submitter supplied) Human transcriptome analysis of U2OS cells treated with nocodazole or DMSO (Control). The gene expression profiling will reveal senescence-associated genes induced upon nocodazole treatment. Our preliminary data show that antimitotic drugs treatment promote post-slippage senescence.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17586

4 Samples

Download data: CEL

(Submitter supplied) To study SKP2 in osteosarcoma, we generated a Skp2 KO line and a Skp2 intact line of transgenic OS mouse models: germline Skp2 knockout (“TKO”: Osx1-Cre;Rb1lox/lox;p53lox/lox;SKP2) versus Skp2 intact (“DKO”: Osx1-Cre;Rb1lox/lox;p53lox/lox). We then performed bulk RNAseq of the SKP2 KO vs SKP2 intact OS.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

6 Samples

Download data: CSV, TAB

(Submitter supplied) Senescent cells no longer divide, but remain metabolically active and secrete an array of cytokines, growth factors, and proteases termed the senescence-associated secretory phenotype (SASP). Previous studies have indicated that the SASP factor IL-1α may be an upstream regular of the SASP. We show here that knockdown of the IL-1α receptor IL-1R results in a sizable reduction of SASP expression late in senescence induction. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

12 Samples

Download data: TXT

(Submitter supplied) To study early events in osteosarcoma genesis murine bone marrow derived mesenchymal stem cells (mMSCs) were studied which generated osteosarcoma-like tumours upon injection into nude mice. The process of transformation was studied in a step-wise manner by culturing the MSCs in vitro for 28 passages. Subsequently the transformed passage 28 MSCs were compared to their normal passage 12 originator cells in a number of experiments to identify underlying events prior to malignant transformation. more...

Organism:

Mus musculus

Type:

Genome variation profiling by genome tiling array

Platform:

GPL4092

7 Samples

Download data: TXT

(Submitter supplied) Analysis of gene changes in different genes modulation in MSCs and compared to primary human osteosarcoma cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

12 Samples

Download data: TXT

(Submitter supplied) Breast cancers can be classified using whole genome expression into distinct subtypes that show differences in patient prognosis. One of these groups, the basal-like carcinomas, are poorly differentiated, highly metastatic, and genomically unstable. These tumors also contain specific genetic alterations with one example being frequent p53 mutations. The loss of the tumor suppressor gene encoded by the retinoblastoma (RB1) locus is a well-characterized occurrence in many tumor types, however, its role in breast cancer is less clear with many reports demonstrating a Loss of Heterozygosity (LOH) that does not correlate with loss of RB1 protein expression. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

4 related Platforms

66 Samples

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(Submitter supplied) Mice have been used as models for human breast cancers for many years, however, it is still unclear which murine models faithfully represent human tumor phenotypes. To address this question, we used DNA microarrays to characterize 10 different murine mammary models and compared these data to the expression patterns from primary human breast tumors. Hierarchical clustering analysis of the murine samples showed that the WAP-Myc, MMTV-Neu, MMTV-PyMT, WAP-Int3, and C3(1)-Tag tumors were highly correlated within each model. more...

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

4 related Platforms

354 Samples

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(Submitter supplied) Gene expression of mouse cells was studied by extracting total RNA from cultured mouse mesenchymal stem cells (MSCs) and zebrafish injected with mouse MSCs, in addition the host response was studied by hybridizing the zebrafish RNA to zebrafish arrays.

Organism:

Danio rerio; Mus musculus

Type:

Expression profiling by array

Platforms:

GPL14629 GPL14628

21 Samples

Download data: TXT

(Submitter supplied) RNA from GL261 (glioma) and LLC1 (Lewis Lung carcinoma) samples were sequenced at a depth of 142M and 188M paired reads, respectively.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

2 Samples

Download data: TXT