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Sample GSM92575 Query DataSets for GSM92575
Status Public on Apr 01, 2006
Title PC xenograft PC324 replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, without expression of a functional androgen receptor, on intact mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) peter.hendriksen@wur.nl
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.09 5318.216667 3702.7375 6006.447552 2851.690236
2 0.21 4648.474576 3688.44186 4283.149606 2811.737313
3 0.75 11060.47917 3731.933333 9808.14359 2896.779026
4 0.05 4661.356322 3675.798489 4503.61165 2796.666667
5 3641.119266 3655.778976 2705.174312 2775.574124
6 3692.741935 3656.487273 2717.129032 2747.047273
7 3677.277778 3679.732673 2786.194444 2750.19802
8 3714.138889 3670.323529 2777.916667 2818.460784
9 3643.559633 3650.164306 2771.889908 2739.915014
10 3687.944954 3667.431981 2814.220183 2757.98568
11 3724.055556 3671.100629 2773.958621 2745.905363
12 3679.5 3643.391892 2822.322581 2785.425676
13 3681.080645 3657.648438 2779.548387 2769.46875
14 3669.348837 3668.185022 2783.790698 2793.788546
15 3652.481481 3657.983827 2824.477064 2753.261456
16 3643.137615 3656.575499 2820.311927 2762.552707
17 3699.522936 3665.524079 2829.027523 2759.569405
18 3666.145161 3673.643454 2822.032258 2765.532033
19 3623.59633 3664.809668 2789.376147 2769.480363
20 3686.073394 3674.371859 2814.385321 2761.417085

Total number of rows: 19185

Table truncated, full table size 1041 Kbytes.




Supplementary data files not provided
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