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Sample GSM92570 Query DataSets for GSM92570
Status Public on Apr 01, 2006
Title PC xenograft PC133 after castration replicate_2
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-independent xenograft, without expression of a functional androgen receptor, 15 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) peter.hendriksen@wur.nl
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.83 4650.208333 1615.658824 8310.866667 3062.802198
2 0.44 2273.350649 1589.973333 3781.691176 3260.886275
3 0.18 4978.596026 1641.612565 6162.509091 3189.094828
4 -0.07 2510.067797 1588.78453 3972.058824 2843.256684
5 1534.348624 1559.728 2787.229358 2862.842667
6 1640.144144 1579.762452 4455.553719 3397.560606
7 1569.431193 1577.044674 2700.348624 2904.848249
8 1533.46789 1548.996721 2675.614679 2867.25
9 1531.758065 1526.774373 2889.483871 2820.373259
10 1534.733945 1550.940741 2842.814815 2968.690476
11 1523.541284 1532.832861 2669.926606 2820.436261
12 1553.806452 1532.972789 2931.806452 2813.156463
13 1553.394495 1527.630662 2760.201835 2761.944251
14 1539.677419 1519.565476 2782.483871 2806.869048
15 1545 1534.719298 2863.248 2807.612069
16 1535.613793 1526.018735 2878.694444 2713.100703
17 1527.387097 1527.435374 2927.691057 2769.29932
18 1535.593023 1544.4 2830.837209 2821.22069
19 1540.366972 1544.394822 2753.688073 2851.508091
20 1535.765517 1527.961353 2861.186207 2859.42029

Total number of rows: 19185

Table truncated, full table size 1040 Kbytes.




Supplementary data files not provided
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