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Sample GSM92545 Query DataSets for GSM92545
Status Public on Apr 01, 2006
Title PC xenograft PC329 replicate_1
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-dependent xenograft, androgen receptor expressing, on intact mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) peter.hendriksen@wur.nl
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 0.23 3846.544554 2441.848921 4223.933333 2235.711111
2 0.33 3054.378378 2434.089172 3058.676259 2212.854489
3 0.79 6297.029412 2494.925926 5589.958115 2316.576419
4 0.3 3246.902439 2413.570313 3366.836957 2203.5
5 2378.550459 2387.630996 2186.66055 2148.309963
6 2403.614679 2388.57732 2188.477064 2162.845361
7 2365.06422 2383.609756 2176.238532 2157.89547
8 2382.370968 2384.826498 2179.709677 2160.07571
9 2384.612903 2376.283912 2153.145161 2171.798107
10 2384.872093 2380.027174 2163.116279 2151.429348
11 2392.911917 2381.134328 2195.968912 2155.391045
12 2382.317241 2378.624606 2192.744828 2162.378549
13 2380.634538 2372.879581 2164.795181 2175.225131
14 2389.322581 2347.984375 2158.83871 2170.6875
15 2382.318841 2362.938272 2180.724638 2163.104938
16 2374.091743 2396.115226 2167.798165 2225.07234
17 2378.715596 2401.862595 2218.844037 2195.806452
18 2371.743119 2382.248521 2173.816514 2185.202454
19 2366.744828 2368.195122 2174.386207 2153.066202
20 2372.477064 2367.090047 2167.46789 2159.478673

Total number of rows: 19185

Table truncated, full table size 1044 Kbytes.




Supplementary data files not provided
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