NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM92544 Query DataSets for GSM92544
Status Public on Apr 01, 2006
Title PC xenograft PC310 after castration replicate_2
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-dependent xenograft, androgen receptor expressing, 14 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) peter.hendriksen@wur.nl
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 -0.25 3843.948052 1551.165049 5771.052632 2177.221154
2 -0.16 2137.889764 1508.485623 3105.051724 2073.481481
3 0.53 5642.62069 1544.79646 5740.054054 2146.428571
4 0.22 2410.624 1507.091525 3247.234783 2086.767213
5 1470.887097 1472.967581 1978.322581 2006.453865
6 2031.037433 1633.862595 2731.271523 2202.590909
7 1486.146789 1488.88961 2006.568807 2055.758308
8 1486.201835 1482.743935 2064.238532 1973.15903
9 1477.759259 1468.4375 1998.157407 1995.368889
10 1464.013793 1469.313433 2004.662069 1944.167164
11 1469.959839 1477.81982 1996.693548 1968.09009
12 1490.899083 1508.377358 2045.555556 2072.952756
13 1481.376147 1471.483544 2035.926606 1960.263291
14 1473.616279 1478.982301 2039.686047 1995.550661
15 1505.117241 1476.974545 2009.903448 1998.894545
16 1484.564516 1474.698254 2043.16129 2019.326683
17 1495.61658 1493.138264 1991.07772 2002.758842
18 1496.965517 1491.857627 1985.696552 2023.627119
19 1481.510417 1486.59164 2043.751295 2019.980707
20 1492.896552 1469.073746 1991.144828 1984.554572

Total number of rows: 19185

Table truncated, full table size 1043 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap