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Sample GSM92540 Query DataSets for GSM92540
Status Public on Apr 01, 2006
Title PC xenograft PC295 after castration replicate_2
Sample type RNA
 
Channel 1
Source name xenograft
Organism Homo sapiens
Characteristics Androgen-dependent xenograft, androgen receptor expressing, 6 days after castration of male host mouse.
Extracted molecule total RNA
Label Cy3
 
Channel 2
Source name common reference of 13 cell lines
Organism Homo sapiens
Characteristics 13 cell lines that originated from prostate cancer (Du145 and LNCaP), breast (MCF7 and ZR75.1), bone (MG63), colon (HT29), endothelium (Ea-hy926), ovary (A2780), skin (SK-MEL-28), B-cell leukemia (Raji), T-cell lymphoblastoma (Jurkat) and fibroblasts (N92-19N and N92-19T).
Extracted molecule total RNA
Label Cy5
 
 
Description The prostate cancer xenografts have been described by van Weerden and Romijn (Prostate 2000; 43:263-271). The xenografts were serially passaged subcutaneously in both shoulders of male Swiss nude mice.
Data processing Normalization was performed using R-routines (Lowess method; http://cmfdb.nki.nl/). All spots with an R-value below 3 or with an average intensity less than 0.4 of the median spot intensity per subarray were considered to be insufficiently reliable and were excluded from the data analysis.
 
Submission date Jan 13, 2006
Last update date Jan 23, 2006
Contact name Peter Hendriksen
E-mail(s) peter.hendriksen@wur.nl
Organization name Wageningen University & Research
Department RIKILT
Street address Akkermaalsbos 2
City Wageningen
ZIP/Postal code 6708 WB
Country Netherlands
 
Platform ID GPL3349
Series (1)
GSE4084 Human prostate cancer xenografts on intact mice or after castration.

Data table header descriptions
ID_REF spot number
VALUE normalized log2 ratio of xenograft / common reference
CH1_SIG_MEAN Mean signal intensity of the spot of channel 1
CH1_BKD_MEAN Mean intensity of the background of channel 1
CH2_SIG_MEAN Mean signal intensity of the spot of channel 2
CH2_BKD_MEAN Mean intensity of the background of channel 2

Data table
ID_REF VALUE CH1_SIG_MEAN CH1_BKD_MEAN CH2_SIG_MEAN CH2_BKD_MEAN
1 0.05 6068.454545 2804.804196 6178.909953 2134.212454
2 0.06 3550.130435 2760.098765 3104.938272 2060.053333
3 0.44 7210.822967 2850.335079 6223.225225 2147.629213
4 0.4 4228.691824 2769.655385 3540.475676 2058.167224
5 2770.023256 2754.410959 2012.046512 2002.138249
6 2782.899083 2762.678112 1997.59633 2013.218884
7 2766.134715 2760.53202 1996.777202 2006.453202
8 2771.02069 2755.791798 1997.158621 1997.722397
9 2779.614679 2766.66899 2005.33945 2006.543554
10 2746 2742.218182 2009.103448 2005.010909
11 2742.993103 2746.807799 2002.662069 2003.927577
12 2752.654971 2743.796226 2008.418605 2022.354717
13 2794.880734 2767.055794 2002.825688 2015.150215
14 2825.743119 2781.118971 2033.027523 2021.03537
15 2813.284404 2776.670025 1997.761468 2009.380353
16 2752.937931 2752.603922 2038.717241 2041.32549
17 2710.550459 2746.756972 2024.036697 2042.143426
18 2744.348624 2741.954416 2031.009174 2038.809117
19 2720.972477 2747.077491 1995.284404 2012.413284
20 2711 2749.50838 2006.697248 2047.195531

Total number of rows: 19185

Table truncated, full table size 1042 Kbytes.




Supplementary data files not provided
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