|
| Status |
Public on Jan 05, 2013 |
| Title |
RA P30_3 |
| Sample type |
RNA |
| |
|
| Source name |
12 months-old_rats_MSCs_passage 30
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
age: 12 months
tissue source: bone marrow
cell type: mesenchymal stromal cells (MSCs)
passage: passage 30
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
| Label |
biotin
|
| Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
SAMPLE 12
|
| Data processing |
Illumina BeadStudio software version 3
|
| |
|
| Submission date |
Mar 19, 2012 |
| Last update date |
Jan 05, 2013 |
| Contact name |
Sven Geissler |
| E-mail(s) |
sven.geissler@charite.de
|
| Organization name |
Charite - Universitaetsmedizin Berlin
|
| Department |
Julius Wolff Institute and BCRT
|
| Lab |
AG Geissler/Ode
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE36596 |
Functional Comparison of Chronological and In Vitro Aging: Differential Role of the Cytoskeleton and Mitochondria in Mesenchymal Stromal Cells |
|
