|
Status |
Public on Jan 05, 2013 |
Title |
RJ P2_3 |
Sample type |
RNA |
|
|
Source name |
3 wks-old_rats_MSCs_passage 2
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague Dawley age: 3 weeks tissue source: bone marrow cell type: mesenchymal stromal cells (MSCs) passage: passage 2
|
Extracted molecule |
total RNA |
Extraction protocol |
The RNA was isolated using RNeasy Mini Kit (Quiagen), and DNase digestion (RNase-free DNase Set, Quiagen) has been applied on the columns during isolation.
|
Label |
biotin
|
Label protocol |
Illumina Gene Expression protocols contain a first- and second-strand reverse transcription step, followed by a single in vitro transcription (IVT) amplification that incorporates biotin-labeled nucleotides. Subsequent steps include array hybridization, washing, blocking, and streptavadin-Cy3 staining. Fluorescence emission by Cy3 is quantitatively detected for downstream analysis.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 3 SAMPLE 3
|
Data processing |
Illumina BeadStudio software version 3
|
|
|
Submission date |
Mar 19, 2012 |
Last update date |
Jan 05, 2013 |
Contact name |
Sven Geissler |
E-mail(s) |
sven.geissler@charite.de
|
Organization name |
Charite - Universitaetsmedizin Berlin
|
Department |
Julius Wolff Institute and BCRT
|
Lab |
AG Geissler/Ode
|
Street address |
Augustenburger Platz 1
|
City |
Berlin |
State/province |
Berlin |
ZIP/Postal code |
13353 |
Country |
Germany |
|
|
Platform ID |
GPL8031 |
Series (1) |
GSE36596 |
Functional Comparison of Chronological and In Vitro Aging: Differential Role of the Cytoskeleton and Mitochondria in Mesenchymal Stromal Cells |
|