|
| Status |
Public on Dec 28, 2011 |
| Title |
sample 4: ASML kd exosomes |
| Sample type |
RNA |
| |
|
| Source name |
exosomes from ASML CD44v kd cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: ASML CD44v kd cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays at DKFZ core facility
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol at DKFZ core facility
|
| Scan protocol |
Standard Illumina scanning protocol at DKFZ core facility
|
| Description |
replicate1
|
| Data processing |
The data were normalised at the DKFZ core facility
|
| |
|
| Submission date |
Dec 27, 2011 |
| Last update date |
Dec 28, 2011 |
| Contact name |
Sanyukta Rana |
| E-mail(s) |
s.rana@uni-heidelberg.de
|
| Organization name |
University Hostipal Heidelberg
|
| Department |
Tumor Cell Biology
|
| Lab |
AG Zoller
|
| Street address |
Im Neuenheimerfeld 365
|
| City |
Heidelberg |
| ZIP/Postal code |
69115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8031 |
| Series (2) |
| GSE34737 |
Comparative analysis of mRNA expression in untreated lymph node stroma cells and upon treatment with exosomes derived from ASMLwt, ASML cd44v knockdown cells. |
| GSE34739 |
Exosomal tumor miRNA modulates premetastatic organ cells |
|
