|
Status |
Public on Jul 21, 2011 |
Title |
HZ7, rep1 |
Sample type |
RNA |
|
|
Source name |
hippocampus, dw HZ, saline, 7 days
|
Organism |
Rattus norvegicus |
Characteristics |
strain: HsdOla:DWARF-dw-4 HZ tissue: hippocampus age: 42 days treatment: saline treatment duration: 7 days sample type: control
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer.
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
5298408026_I Replicate 1 of 4.
|
Data processing |
The data were normalized using quantile normalization in GenomeStudio v2010.2.
|
|
|
Submission date |
May 24, 2011 |
Last update date |
Jul 21, 2011 |
Contact name |
Penn State COM |
Organization name |
Penn State College of Medicine
|
Department |
Genome Sciences Facility
|
Street address |
500 University Drive
|
City |
Hershey |
State/province |
PA |
ZIP/Postal code |
17033 |
Country |
USA |
|
|
Platform ID |
GPL8031 |
Series (1) |
GSE29512 |
Circulating insulin-like growth factor (IGF)-1 Regulates Hippocampal IGF-1 Levels and Brain Gene Expression During Adolescence |
|