|
| Status |
Public on Jul 21, 2011 |
| Title |
dw/dw+GH7, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, dw/dw, GH, 7 days
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: HsdOla:DWARF-dw-4
tissue: hippocampus
age: 42 days
treatment: growth hormone (GH)
treatment duration: 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
5298408026_E
Replicate 1 of 4.
|
| Data processing |
The data were normalized using quantile normalization in GenomeStudio v2010.2.
|
| |
|
| Submission date |
May 24, 2011 |
| Last update date |
Jul 21, 2011 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE29512 |
Circulating insulin-like growth factor (IGF)-1 Regulates Hippocampal IGF-1 Levels and Brain Gene Expression During Adolescence |
|
