|
| Status |
Public on Oct 30, 2011 |
| Title |
Impaired 1 |
| Sample type |
RNA |
| |
|
| Source name |
hippocampus, cognitively impaired
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fischer 344 x Brown Norway (F1)
tissue: hippocampus
cell type: synaptosomes
age: 28 months
group: aged cognitively impaired
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
5022655028_C
Replicate 1 of 7.
|
| Data processing |
The data were normalized using quantile normalization in GenomeStudio v1.0.6.
|
| |
|
| Submission date |
May 24, 2011 |
| Last update date |
Oct 30, 2011 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE29511 |
Induction of the neuronal major histocompatibility complex class I and paired immunoglobulin-like receptor B pathway in the hippocampus with aging and cognitive decline |
|
