|
Status |
Public on Oct 30, 2011 |
Title |
Intact 6 |
Sample type |
RNA |
|
|
Source name |
hippocampus, cognitively intact
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Fischer 344 x Brown Norway (F1) tissue: hippocampus cell type: synaptosomes age: 28 months group: aged cognitively intact
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with the Agilent Bioanalyzer.
|
Label |
Cy3
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
5022655028_A Replicate 1 of 5.
|
Data processing |
The data were normalized using quantile normalization in GenomeStudio v1.0.6.
|
|
|
Submission date |
May 24, 2011 |
Last update date |
Oct 30, 2011 |
Contact name |
Penn State COM |
Organization name |
Penn State College of Medicine
|
Department |
Genome Sciences Facility
|
Street address |
500 University Drive
|
City |
Hershey |
State/province |
PA |
ZIP/Postal code |
17033 |
Country |
USA |
|
|
Platform ID |
GPL8031 |
Series (1) |
GSE29511 |
Induction of the neuronal major histocompatibility complex class I and paired immunoglobulin-like receptor B pathway in the hippocampus with aging and cognitive decline |
|