|
| Status |
Public on Apr 28, 2011 |
| Title |
NSC miR-128 3 |
| Sample type |
RNA |
| |
|
| Source name |
mNSC nucleofected with miR-128l, replicate
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Neuronal Stem Cells
developmental stage: E14.5
strain: CD-1 albino
|
| Treatment protocol |
mouse NSCs were nucleofected either with miR-128 mimic or miR-Control
|
| Growth protocol |
mouse NSCs were grown as neurospheres and in proliferation conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by Rneasy Column purification
|
| Label |
biotin
|
| Label protocol |
One μg of total RNA was labeled following the GeneChip® Whole Transcript (WT) Sence Target Labeling Protocol (P/N 701880)
|
| |
|
| Hybridization protocol |
GeneChip® Hybridization, Wash and Stain Kit was used to prepare samples for hybridization to the Gene 1.0 ST Array. More information about this protocol can be found on the Affymetrix website.
|
| Scan protocol |
Image analysis was performed using the standard Affymetrix protocols (GeneChip® Expression Wash, Stain and Scan User Manual (P/N 702731)). Scanning was performed using the GeneChip® Scanner 3000 and Affymetrix® GeneChip® Command Console® (AGCC).
|
| Data processing |
The raw CEL files were imported into R using the 'affy' package. Arrays were processed using RMA background correction, quantile normalization, and median polish summarization using only the perfect match (PM) probes.
|
| |
|
| Submission date |
Apr 27, 2011 |
| Last update date |
Apr 28, 2011 |
| Contact name |
Rachid Karam |
| E-mail(s) |
rkaram@ucsd.edu
|
| Phone |
8588224701
|
| Fax |
8588211966
|
| Organization name |
UCSD
|
| Department |
Reproductive Medicine
|
| Lab |
Miles Wilkinson Lab
|
| Street address |
9500 Gilman Dr. #0864
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platform ID |
GPL13455 |
| Series (1) |
| GSE28889 |
Differentially expressed genes and transcript isoforms in mouse NSCs expressing miR-128 |
|
