|
| Status |
Public on Dec 31, 2005 |
| Title |
KFr13Tx |
| Sample type |
RNA |
| |
|
| Source name |
ovarian cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
serous adenoarcinoma
TXL/CDDP-resistant variant of KF28
|
| Biomaterial provider |
National Defense Medical College, Saitama, Japan
|
| Growth protocol |
Cultured in RPMI 1640 medium (Life Technologies, Inc., Grand Island, NY) containing 10% heat-inactivated fetal bovine serum (FBS; BioWhittaker, Verviers, Belgium) at 37°C in a humidified atmosphere of 5% CO2 and passaged every 3 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using QIAGEN RNeasy mini kit (QIAGEN, Inc., Valencia, CA). The quality of RNA was checked using Agilent Technologies 2100 Bioanalyzer (Agilent, Palo Alto, CA).
|
| Label |
Cy5
|
| Label protocol |
Biotin-labeled cRNA was synthesized from 1-µg total RNA using CodeLink Expressioin Assay Reagent Kit (Amersham) and Biotin-11-UTP (PerkinElmer) according to the manufacturers’ protocols.
|
| |
|
| Hybridization protocol |
Each cRNA sample was fragmented, and hybridized with a CodeLink UniSet Human 20K I Bioarray™ using Parallel Processing Kit™ (CodeLink) for 18 h at 37°C in an Innova™ 4080 incubator (New Brunswick Scientific) shaking at 300 rpm. The hybridized arrays were rinsed with 0.75 x TNT buffer at 46°C for 1 h, labeled with Streptavidin-Cy5 at room temperature for 30 min, rinsed with TNT buffer and 0.05% Tween 20, and dried by centrifugation.
|
| Scan protocol |
The arrays were scanned using Agilent DNA Microarray Scanner™ (Agilent, PaloAlto, CA, USA).
|
| Description |
Expression profiles of exponentially growing ovarian cancer cell lines were compared with their drug sensitivity.
|
| Data processing |
Tiff file was analyzed with CodeLink Expression Analysis Software (Ver. 2.3).
|
| |
|
| Submission date |
Jul 25, 2005 |
| Last update date |
Oct 28, 2005 |
| Contact name |
Keiko Hiyama |
| E-mail(s) |
khiyama@hiroshima-u.ac.jp
|
| Phone |
81-82-257-5841
|
| Fax |
81-82-256-7105
|
| Organization name |
Hiroshima University
|
| Department |
Research Institute for Radiation Biology and Medicine
|
| Lab |
Dept. Translational Cancer Research
|
| Street address |
1-2-3 Kasumi, Minami-ku
|
| City |
Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
734-8553 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1928 |
| Series (1) |
| GSE3001 |
Chemosensitivity prediction of Paclitaxel-Platinum combination therapy by expression profile in ovarian cancer |
|
