|
| Status |
Public on Jun 05, 2010 |
| Title |
Mutant thoracic spinal cord 24 hours after SCI |
| Sample type |
RNA |
| |
|
| Source name |
Rat spinal cord
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: thoracic spinal cord
genotype: G93A SOD1
protocol: 24 hours after SCI
strain: Sprague-Dawley
age: 10 weeks
geneder: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the thoracic spinal cord samples obtained from injured and uninjured rats, and from animals subjected to laminectomy, using the SV total RNA isolation system (Promega, UK). RNA quantification was performed using a Nanodrop ND-1000 spectrophotometer and quality was checked using the Agilent bioanalyser system (Agilent).
|
| Label |
biotin
|
| Label protocol |
cRNA labeling was performed with 750 ng of pooled RNA, using the Ambion Total Prep kit. cRNA purity and labelling was checked using the Nanodrop and Agilent bioanalyser.
|
| |
|
| Hybridization protocol |
500 ng from each of the 12 RNA pools were hybridised simultaneously to 12 arrays, using a standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol, Illumina Bead-Chip Reader
|
| Description |
thoracic cord
|
| Data processing |
Output files were processed using the Gene Expression BeadStudio 3.2.6, with background subtraction
|
| |
|
| Submission date |
Jun 04, 2010 |
| Last update date |
Jun 04, 2010 |
| Contact name |
ANDREA MALASPINA |
| E-mail(s) |
a.malaspina@qmul.ac.uk
|
| Phone |
+44 (0)20 7882 2483
|
| Fax |
+44 (0)20 7882 2200
|
| Organization name |
Institute of Cell and Molecular Science
|
| Department |
Neuroscience
|
| Lab |
Molecular Neurobiology
|
| Street address |
4 Newark Street
|
| City |
London |
| State/province |
England |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE22161 |
Comparative gene expression analysis of thoracic spinal cord from G93A SOD1 mutant rats and from wild type littermates following mild compression injury |
|
