|
| Status |
Public on Mar 16, 2010 |
| Title |
Mutant lumbar spinal cord disease-onset |
| Sample type |
RNA |
| |
|
| Source name |
Rat spinal cord
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
age: 10 weeks
gender: female
genotype: G93A SOD1
tissue: lumbar spinal cord
disease state: disease-onset
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from the spinal cord samples using the SV total RNA isolation system (Promega, UK). RNA quantification was performed using a Nanodrop ND-1000 spectrophotometer and quality was checked using the Agilent bioanalyser system (Agilent).
|
| Label |
biotin
|
| Label protocol |
cRNA labeling was performed with 750 ng of pooled RNA, using the Ambion Total Prep kit. cRNA purity and labelling was checked using the Nanodrop and Agilent bioanalyser.
|
| |
|
| Hybridization protocol |
500 ng from each of the RNA pools were hybridised simultaneously to Brad-chip arrays, using a standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol, Illumina Bead-Chip Reader
|
| Description |
thoracic cord
|
| Data processing |
Output files were processed using the Gene Expression BeadStudio 3.2.6, with background subtraction
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Mar 15, 2010 |
| Contact name |
ANDREA MALASPINA |
| E-mail(s) |
a.malaspina@qmul.ac.uk
|
| Phone |
+44 (0)20 7882 2483
|
| Fax |
+44 (0)20 7882 2200
|
| Organization name |
Institute of Cell and Molecular Science
|
| Department |
Neuroscience
|
| Lab |
Molecular Neurobiology
|
| Street address |
4 Newark Street
|
| City |
London |
| State/province |
England |
| ZIP/Postal code |
E1 2AT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE20894 |
Gene expression and gene ontology analysis of the transcriptional changes occurring in spinal cord from the G93A SOD1 gene mutant rat |
|
