|
| Status |
Public on Jun 01, 2010 |
| Title |
STZ 4 |
| Sample type |
RNA |
| |
|
| Source name |
Retina, STZ
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
gender: male
tissue: retina
treatment: streptozotocin (STZ)
|
| Treatment protocol |
Diabetes was induced in Sprague-Dawley male rats (Charles River Laboratories, Wilmington, MA) by intraperitoneal injection of 65 mg/kg streptozotocin (STZ) (Sigma-Aldrich, St. Louis, MO) in 10mM sodium citrate pH 4.5 vehicle. Control rats were injected with an equal dose of vehicle only.
|
| Growth protocol |
Rats had free access to food and water, and were maintained on a 12 hour light/dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol. Cy3-Streptavidin was incorporated during the Wash and Block protocol as a fluor for scanning.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
Diabetes was induced by intraperitoneal injection of STZ.
Replicate 4 of 4.
1685398124_I
|
| Data processing |
The data were normalized using quantile normalization in GenomeStudio v1.0.6.
|
| |
|
| Submission date |
Mar 15, 2010 |
| Last update date |
Apr 21, 2010 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE20886 |
Multi-modal proteomic target discovery and orthogonol confirmation of preclinical diabetic retinopathy drug development biomarkers |
|
