|
| Status |
Public on Jun 01, 2010 |
| Title |
Control4 |
| Sample type |
RNA |
| |
|
| Source name |
Control Retina
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: retina
treatment group: control
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 4
|
| Data processing |
Data was normalized in BeadStudio 2.0 using Cubic Spline normalization method. The data was exported and then imported into Genespring 7.3 where it was filtered on expression levels and p-value detection. Volcano plot between the 2 groups using fold change and p-values was used to find the significant genelist.
|
| |
|
| Submission date |
Feb 24, 2010 |
| Last update date |
Mar 23, 2010 |
| Contact name |
Penn State COM |
| Organization name |
Penn State College of Medicine
|
| Department |
Genome Sciences Facility
|
| Street address |
500 University Drive
|
| City |
Hershey |
| State/province |
PA |
| ZIP/Postal code |
17033 |
| Country |
USA |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE20521 |
Vascular Permeability and Apoptosis are Separable Processes in Retinal Ischemia-Reperfusion Injury |
|
