|
| Status |
Public on Dec 28, 2009 |
| Title |
whole lung Fisher rat at PN14 replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
rat whole lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Fisher
age (pn postnatal days): PN14
tissue: whole lung
beadchip sentrix barcode: 1814671020
beadchip section: D
microarray hybridization date: 08/07/07
number of detected genes (p<0.01): 10000
number of detected genes (p<0.05): 11804
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA from whole lung tissue and primary lung cells was isolated using Trizol reagent (Invitrogen, Burlington, ON, Canada) according to the manufacturer’s instructions. RNA was resuspended in 1x RNASecure (Ambion, Austin, TX, USA) and treated with the Turbo DNase-free kit (Ambion, Austin, TX, USA) to remove any traces of DNA.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
whole lung Fisher rat at PN14 replicate 1
|
| Data processing |
The data were standard background corrected and quantile normalized using the R Bioconductor package lumi.
|
| |
|
| Submission date |
Dec 28, 2009 |
| Last update date |
Dec 28, 2009 |
| Contact name |
Alvin T. Kho |
| E-mail(s) |
alvin_kho@hms.harvard.edu
|
| Phone |
617-919-2182
|
| Organization name |
Boston Children's Hospital
|
| Department |
Informatics Program
|
| Street address |
320 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE19666 |
Genetic Influences on Asthma Susceptibility in Developing Lung |
|
