|
| Status |
Public on May 10, 2011 |
| Title |
Liver biopsy from GBV-C negative subject, 3 |
| Sample type |
RNA |
| |
|
| Source name |
liver, GBV-C negative
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: liver
gbv-c virus status: negative
hiv virus status: positive
hcv virus status: positive
hcv genotype: 1
gender: male
|
| Treatment protocol |
Ultrasound-guided liver biopsy was performed to obtain liver tissue samples from patients.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was collected into RNA later and then extracted using RNAeasy MiniKit (Qiagen, Dusseldorf, Germany).
|
| Label |
biotin
|
| Label protocol |
5-15ug of purified total RNA from each patient was labelled with biotin as recommended in the Affymetrix GeneChip Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
Affymetrix fluidics station following protocol EukGE-WS2v5_450.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner M10.
|
| Description |
LB57G701
|
| Data processing |
1-way ANOVA was used to detect genes differentially expressed between GBV-C positive and negative patients using Partek Genomics suite and applying a custom CDF file (Hs133P_Hs_UG_8), available from Molecular and Behavioural Neuroscience Institute, University of Michigan. RMA was used to generate expression values.
|
| |
|
| Submission date |
Jun 12, 2009 |
| Last update date |
May 10, 2011 |
| Contact name |
Victoria Mary Perreau |
| E-mail(s) |
vperreau@unimelb.edu.au
|
| Phone |
+61 3 8344 1835
|
| Organization name |
University of Melbourne
|
| Department |
Center for Neuroscience
|
| Street address |
Parkville
|
| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3010 |
| Country |
Australia |
| |
|
| Platform ID |
GPL8715 |
| Series (1) |
| GSE16593 |
Differential expression associated with GB virus C in HCV/HIV co-infection |
|
