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Sample GSM3509476 Query DataSets for GSM3509476
Status Public on Dec 13, 2018
Title JFE011: BM37 Ind
Sample type RNA
 
Source name Engineered human adipose tissue
Organism Homo sapiens
Characteristics tissue: Bone marrow-derived human stromal/stem cells differentiated into adipocyte tissue
Growth protocol Cells were differenciated or not into adipocytes according to the protocol described in the associated publication. Reconstructed tissues were analysed at day 35 of culture.
Extracted molecule total RNA
Extraction protocol RNA was extracted with RNeasy Lipid tissue Mini Kit (Qiagen), followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Data processing The data were normalised using quantile normalisation with IlluminaGUI in R
 
Submission date Dec 12, 2018
Last update date Dec 13, 2018
Contact name Julie Fradette
E-mail(s) julie.fradette@fmed.ulaval.ca
Organization name Université Laval
Department Chirurgie
Lab LOEX - Julie Fradette
Street address 1401 18e Rue
City Québec
State/province Québec
ZIP/Postal code G1J 1Z4
Country Canada
 
Platform ID GPL6097
Series (1)
GSE123685 Genome-wide analysis of human reconstructed adipose tissue, human reconstructed connective tissue and native fat

Supplementary data files not provided
Processed data are available on Series record

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