|
| Status |
Public on Jul 11, 2008 |
| Title |
E2F3 +/- Involution Day 4 rep3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mammary Gland
|
| Organism |
Mus musculus |
| Characteristics |
E2F3 +/- Involution Day 4 rep3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Briefly, mammary gland RNA was prepped through CsCl ultracentrafugation.
|
| Label |
Cy5
|
| Label protocol |
Abion_AmpII protocol (attached below) (from Duke Institute fro Genome Sciences and Policy Microarray Facility) http://www.genome.duke.edu/cores/microarray/services/spotted-arrays/protocols/
|
| |
|
| Channel 2 |
| Source name |
Total RNA from mouse reference library
|
| Organism |
Mus musculus |
| Characteristics |
reference
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from mouse reference library
|
| Label |
Cy3
|
| Label protocol |
Abion_AmpII protocol (attached below) (from Duke Institute fro Genome Sciences and Policy Microarray Facility) http://www.genome.duke.edu/cores/microarray/services/spotted-arrays/protocols/
|
| |
|
| |
| Hybridization protocol |
protocol attached below (from Duke Institute fro Genome Sciences and Policy Microarray Facility) http://www.genome.duke.edu/cores/microarray/services/spotted-arrays/protocols/
|
| Scan protocol |
Axon Genepix Scanner (Genepix 4000B)
|
| Description |
Three mice were taken for each genotype at each timepoint (Involution day 0, 1, 2, 3 and 4) and RNA was prepared from the mammary glands.
|
| Data processing |
Data was analysed with GeneSpring 7 data software package (Silicon Genetics). Each data set was normalised using Lowess intensity-dependent normalisation and then subject to ANOVA. ANOVA was used to eliminate genes that had no signal call in one (or more) of the repeats after Lowess normalization at each of the time points.
|
| |
|
| Submission date |
Apr 04, 2008 |
| Last update date |
Jul 11, 2008 |
| Contact name |
Eran Andrechek |
| Phone |
517-884-5042
|
| Organization name |
Michigan State University
|
| Department |
Physiology
|
| Lab |
Andrechek
|
| Street address |
2120 BPS Building
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL3222 |
| Series (1) |
| GSE11066 |
Expression data from an involution time course in wild type and E2F3 +/- mice. |
|
