|
| Status |
Public on May 04, 2016 |
| Title |
GFP-HMEC |
| Sample type |
RNA |
| |
|
| Source name |
immortalized mammary epithelial cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HMEC
cell type: mammary epithelial cells
|
| Treatment protocol |
Growth the cell in MCF-10A medium or generate the tumor using EBV-HMEC-R or GFP-HMEC-R
|
| Growth protocol |
Cells were cultured in MCF-10A medium with Insulin, EGF, Hydrocortisone, Cholera Toxin, 5% hores serum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the total RNA isolation mini kit (Agilent), RNA quality and concentration were assessed using Nanodrop 1000 and agarose gel electrophoresis. Only RNA samples with absorbance ratio 260/280 > 1.8, preserved rRNA ratio (28S/18S) and no signs of degradation were used.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChipåºHuman Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 and hybridized for 16 hr in the Affymetrix Hybridization Oven 640.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
gene expression data from described sample
|
| Data processing |
Data were analyzed with Expression Console (Affymetrix) and normalized by RMA method.
|
| |
|
| Submission date |
May 03, 2016 |
| Last update date |
May 04, 2016 |
| Contact name |
Gerburg M Wulf |
| E-mail(s) |
gwulf@bidmc.harvard.edu
|
| Phone |
6173317772
|
| Organization name |
Beth Israel Deaconess Medical Center
|
| Street address |
330 Brookline Ave
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL13158 |
| Series (1) |
| GSE81068 |
Expression profile of Epstein Barr Virus infected mammary epithelial cells and Breast tumors |
|
