|
| Status |
Public on Jan 16, 2007 |
| Title |
Normal prostate tissue free of any pathological alteration from organ donor PD012 PD012U95C |
| Sample type |
RNA |
| |
|
| Source name |
PD012, normal prostate tissue free of any pathological alteration from organ donor PD012
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue:normal prostate tissue free of any pathological alteration from brain-dead organ donor
Age: 16
Race: Caucasian
|
| Treatment protocol |
Donor prostate tissues were obtained at the time of organ donation in brain-dead men. Samples were collected from peripheral zone of the prostate gland with at least 60% glandular components and free of any pathological alteration.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Qiagen Rneasy kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U95C array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from normal prostate tissues free of any pathological alteration
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 200.
|
| |
|
| Submission date |
Dec 22, 2006 |
| Last update date |
Jan 16, 2007 |
| Contact name |
Federico Alberto Monzon |
| E-mail(s) |
famonzon@tmhs.org
|
| Organization name |
The Methodist Hospital
|
| Department |
Pathology
|
| Lab |
Molecular Diagnostics
|
| Street address |
6565 Fannin St, MS205
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL93 |
| Series (2) |
| GSE6604 |
Expression data from Normal Prostate Tissue free of any pathological alteration |
| GSE6919 |
Expression Data from Normal and Prostate Tumor Tissues |
|
