|
| Status |
Public on Oct 27, 2012 |
| Title |
Skeletal Muscle_Diabetes |
| Sample type |
RNA |
| |
|
| Source name |
Skeletal Muscle_Diabetes
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague–Dawley
age: 8 weeks
tissue: Skeletal Muscle
|
| Treatment protocol |
The type 2 diabetic model group was injected intraperitoneally with a low dose of STZ (30 mg/kg).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Oct 25, 2012 |
| Last update date |
Oct 27, 2012 |
| Contact name |
xl tang |
| E-mail(s) |
xltangmail@163.com
|
| Organization name |
Nanchang university
|
| Street address |
Xuefu road
|
| City |
Nanchang |
| ZIP/Postal code |
330008 |
| Country |
China |
| |
|
| Platform ID |
GPL8031 |
| Series (1) |
| GSE41832 |
Gene Expression Profiles in Skeletal Muscle and Liver of Type 2 Diabetes Rats |
|
