|
Status |
Public on Oct 27, 2012 |
Title |
Liver_Diabetes |
Sample type |
RNA |
|
|
Source name |
Liver_Diabetes
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague–Dawley age: 8 weeks tissue: Liver
|
Treatment protocol |
The type 2 diabetic model group was injected intraperitoneally with a low dose of STZ (30 mg/kg).
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Oct 25, 2012 |
Last update date |
Oct 27, 2012 |
Contact name |
xl tang |
E-mail(s) |
xltangmail@163.com
|
Organization name |
Nanchang university
|
Street address |
Xuefu road
|
City |
Nanchang |
ZIP/Postal code |
330008 |
Country |
China |
|
|
Platform ID |
GPL8031 |
Series (1) |
GSE41832 |
Gene Expression Profiles in Skeletal Muscle and Liver of Type 2 Diabetes Rats |
|