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| Status |
Public on Apr 07, 2015 |
| Title |
Response of IRF7-deficient peripheral blood mononuclear cells to pH1N1 influenza virus infection |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
IRF7 plays a critical role in the production and amplification of the antiviral type I and III interferon response. Autosomal recessive IRF7-deficiency resulted in life-threatening influenza disease in a 3-year-old child. We studied the impact of IRF7-deficiency in non-hematopoietic tissues (fibroblasts and lung epithelial cells) as well in hematopoietic cells (peripheral blood mononuclear cells (PBMCs)). Genome-wide gene expression analysis demonstrated a profound loss of type I and III IFNs in PBMCs infected with influenza virus.
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| Overall design |
PBMCs were isolated from 4 healthy donors and patients with deficiencies for IRF7 and UNC93B. The cells were infected with influenza virus A/CA/4/2009 at a multiplicity of infection (MOI) of 2 for 8 and 16 hours. Uninfected cells were cultured for 16 hours.
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| Contributor(s) |
Ciancanelli MJ, Huang SX, Luthra P, Garner H, Itan Y, Volpi S, Lafaille FG, Trouillet C, Schmolke M, Albrecht RA, Israelsson E, Lim H, Casadio M, Hermesh T, Lorenzo L, Leung LW, Pedergnana V, Boisson B, Okada S, Picard C, Ringuier B, Troussier F, Chaussabel D, Abel L, Pellier I, Notarangelo LD, Garcia-Sastre A, Basler CF, Geissmann F, Zhang S, Snoeck H, Casanova J |
| Citation(s) |
25814066 |
| Submission date |
Mar 03, 2015 |
| Last update date |
Jan 03, 2024 |
| Contact name |
Stephanie Osmond |
| E-mail(s) |
sosmond@benaroyaresearch.org
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| Organization name |
Benaroya Research Institute
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| Street address |
1201 9th Ave
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| City |
Seattle, |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA277057 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE66486_RAW.tar |
26.2 Mb |
(http)(custom) |
TAR |
| GSE66486_non_normalized.txt.gz |
5.7 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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