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Series GSE6467 Query DataSets for GSE6467
Status Public on Dec 13, 2006
Title Twelve weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA).
Organism Mus musculus
Experiment type Expression profiling by array
Summary Atypical antipsychotic Clozapine has a superior antipsychotic and antimanic effects compared to other antisphicotics. Its widespread use was limited by the side effects of agranulocytosis, cardiomyopathy and metabolic anomalies such as weight gain, and diabetes. Very little is known about mechanisms by which Clozapine works. The aim of this experiment is to compare the chronic gene expression profile of the atypical antipsychotic Clozapine to the typical antipsychotic drug Haloperidol using gene expression Microarray in order to understand the intercellular mechanism behind the therapeutic and the toxic effects of Clozapine.
Keywords: drug response
 
Overall design The study was designed to compare the chronic therapeutic and toxic expression profile of Clozapine to Haloperidol in the mouse brain. All experiments were performed in male C57BL mice at four weeks of age (Biological services, University Collage London). Several theoretical and practical considerations influenced the final experimental design. To avoid the effect of injections on genes’ expression and to simulate the clinical scenario in human, both drugs were applied to the animals’ drinking water using the maximum human therapeutic dose (i.e. 1.6mg/kg/day for Haloperidol and 12mg/kg/day for Clozapine). Thirty animals were divided equally between three treatment groups and received either Haloperidol (10 animals), Clozapine (10 animals) or no treatment (10 animals) for 12 weeks. After twelve weeks, the total RNA from the right forebrains were extracted and hybridized to the Affymetrix MOE430A array. In all the Microarray experiments we have avoided pooling and each RNA sample was an independent biological replicate. The total numbers of used arrays were 30 Affymetrix MOE430A arrays.
 
Contributor(s) Rizig MA, Gurling HM
Citation(s) 22767372
Submission date Dec 06, 2006
Last update date Jan 08, 2019
Contact name Mie A Rizig
E-mail(s) M.rizig@ucl.ac.uk, Dr_mierizig@hotmail.com
Phone +44 (0)2076799436
Fax +44 (0)2076799437
Organization name University Collage London
Department Mental Health Sciences
Lab Molecular Psychiatry Lab
Street address Windeyer Institute of Medical Sciences,46 Cleveland Street
City London
ZIP/Postal code W1T 4JF
Country United Kingdom
 
Platforms (1)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
Samples (30)
GSM148657 Clozapine treated 12weeks AffyGC RMA rep1
GSM148658 Clozapine treated 12weeks AffyGC RMA rep2
GSM148659 Clozapine treated 12weeks AffyGC RMA rep3
This SubSeries is part of SuperSeries:
GSE6512 Clozapine and Haloperidol effect on the mouse brain
Relations
BioProject PRJNA104237

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE6467_RAW.tar 93.1 Mb (http)(custom) TAR (of CEL)

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