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| Status |
Public on Jan 10, 2007 |
| Title |
IFN-gamma activation of cultured neonatal rat microglia |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
IFN-gamma is a classical microglial stimulant. We used microarrays to investigate the microglial gene regulatory network activated by interferon-gamma.
Keywords: Microglia activation
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| Overall design |
Primary rat microglia cultures were established and maintained for 15 days. For activation studies, fresh media containing IFN-gamma (100 U/ml) were added and left overnight (16 hours). Total RNA was extraction and hybridized on Affymetrix microarrays (RG_U34A). Five arrays were run from total RNA derived from unstimulated 5 independent cultures (Mgl_CON1, Mgl_CON2, Mgl_CON3, Mgl_CON5 and Mgl_CON6). Three standard microglial cultures were stimulated (Mgl_IFNgamma_2 and Mgl_IFNgamma_3) with one of the samples analysed in triplicate as a technical control (Mgl_IFNgamma_1a, Mgl_IFNgamma_1b and Mgl_IFNgamma_1c).
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| Contributor(s) |
Moran LB, Duke D, Graeber MB |
| Citation(s) |
17188367 |
| Submission date |
Nov 22, 2006 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Linda B Moran |
| Organization name |
Imperial College London
|
| Department |
Neuropathology
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| Street address |
Fulham Palace Road
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| City |
London |
| ZIP/Postal code |
W6 8RF |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL85 |
[RG_U34A] Affymetrix Rat Genome U34 Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA99625 |
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