|
| Status |
Public on Dec 15, 2006 |
| Title |
The activation of inflammatory response genes by LPS is blocked by HDAC inhibitor Trichostatin A (TSA) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
TSA treatment blocks LPS-induction of several inflammatory target genes in primary macrophages. The microarray experiment was performed to identify LPS-inducible, LXR-sensitive target genes that retained LPS-induction in the presence of TSA.
Thioglycollate-elicited macrophages were isolated from mice by peritoneal lavage 3 days following peritoneal
injection of 2.5 ml 3% thioglycollate (DIFCO). Cells were plated in
RPMI medium 1640 and 10% fetal bovine serum, washed after 5 h the
medium was removed and cells were fed with fresh medium containing
0.5% fetal bovine serum. Cells were treated with TSA (Wako) at 100nM and/or with LPS (Sigma) at
concentration of 100 ng/ml in the absence or
presence of receptor-specific agonist for LXR (GW3965) at 1 µM.
Keywords: TSA, LPS, LXR agonist
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| |
|
| Overall design |
six samples were analyzed
|
| |
|
| Contributor(s) |
ogawa s, benner c, glass c |
| Citation(s) |
17218271 |
| Submission date |
Nov 22, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Christopher Glass |
| E-mail(s) |
ckg@ucsd.edu
|
| Phone |
858-534-6011
|
| Organization name |
University of California, San Diego
|
| Department |
CMM
|
| Lab |
Glass Lab
|
| Street address |
9500 Gilman Dr.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1310 |
Amersham CodeLink UniSet Mouse I Bioarray |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA99623 |
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