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| Status |
Public on Dec 01, 2006 |
| Title |
Expression data from skeletal muscle of young, old and old calorie restricted mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated age-related changes in the transcriptional profile of skeletal muscle in 5 month old (young) and 25 month old (old) C57BL/6NHsd mice using high density oligonucleotide arrays (22,690 transcripts probed). We identified 712 transcripts that are differentially expressed in young (5 month old) and old (25-month old) mouse skeletal muscle. Caloric restriction (CR) completely or partially reversed 87% of the changes in expression. Examination of individual genes revealed a transcriptional profile indicative of increased p53 activity in the older muscle. To determine whether the increase in p53 activity is associated with transcriptional activation of apoptotic targets, we performed RT-PCR on four well known mediators of p53-induced apoptosis: puma, noxa, tnfrsf10b and bok. Expression levels for these proapoptotic genes increased significantly with age (P<0.05), while CR significantly lowered expression levels for these genes as compared to control fed old mice (P<0.05). Age-related induction of p53-related genes was observed in multiple tissues, but was not observed in SOD2+/- and GPX4+/- mice, suggesting that oxidative stress does not mediate the observed age-related increase in expression. Western blot analysis confirmed that protein levels for both p21 and GADD45a, two established transcriptional targets of p53, were higher in the older muscle tissue. These observations support a role for p53-mediated apoptotic activity in mammalian aging.
Keywords: aging, calorie restriction, muscle, p53
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| Overall design |
Animals were killed by cervical dislocation. Five different animals were used for each experimental group. All collected gastrocnemius tissue was then dissected, placed in a microcentrifuge tube, flash-frozen in liquid nitrogen, and stored at -80°C until processed for microarrays.
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| Contributor(s) |
Edwards MG, Anderson RM, Yuan M, Kendziorski CM, Weindruch R, Prolla TA |
| Citation(s) |
17381838 |
| Submission date |
Nov 20, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Michael Edwards |
| E-mail(s) |
michael.edwards@ucdenver.edu
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| Phone |
303-724-6054
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| Organization name |
UC Denver
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| Department |
Pulmonary
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| Street address |
12700 East 19th Avenue, Box C272
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| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (15)
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| GSM146340 |
Gastrocnemius tissue at time 0 from young mouse, biological rep1 |
| GSM146341 |
Gastrocnemius tissue at time 0 from young mouse, biological rep2 |
| GSM146342 |
Gastrocnemius tissue at time 0 from young mouse, biological rep3 |
| GSM146343 |
Gastrocnemius tissue at time 0 from young mouse, biological rep4 |
| GSM146344 |
Gastrocnemius tissue at time 0 from young mouse, biological rep5 |
| GSM146345 |
Gastrocnemius tissue at time 0 from aged mouse, biological rep1 |
| GSM146346 |
Gastrocnemius tissue at time 0 from aged mouse, biological rep2 |
| GSM146347 |
Gastrocnemius tissue at time 0 from aged mouse, biological rep3 |
| GSM146348 |
Gastrocnemius tissue at time 0 from aged mouse, biological rep4 |
| GSM146349 |
Gastrocnemius tissue at time 0 from aged mouse, biological rep5 |
| GSM146350 |
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep2 |
| GSM146351 |
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep3 |
| GSM146352 |
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep5 |
| GSM146353 |
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep6 |
| GSM146354 |
Gastrocnemius tissue at time 0 from aged calorie restricted mouse, biological rep7 |
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| Relations |
| BioProject |
PRJNA100587 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE6323_RAW.tar |
49.4 Mb |
(http)(custom) |
TAR (of CEL) |
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