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| Status |
Public on Oct 29, 2006 |
| Title |
Expression differences in the liver of a congenic mouse with low serum IGF-1 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Several studies have shown that bone mineral density (BMD), a clinically measurable predictor of osteoporotic fracture, is the sum of genetic and environmental influences. In addition, serum IGF-1 levels have been correlated to both BMD and fracture risk. We previously identified a Quantitative Trait Locus (QTL) for Bone Mineral Density (BMD) on mouse Chromosome (Chr) 6 that overlaps a QTL for serum IGF-1. The B6.C3H-6T (6T) congenic mouse is homozygous for C57BL/6J (B6) alleles across the genome except for a 30 cM region on Chr 6 that is homozygous for C3H/HeJ (C3H) alleles. This mouse was created to study biology behind both the BMD and the serum IGF-1 QTLs and to identify the gene(s) underlying these QTLs. Female 6T mice have lower BMD and lower serum IGF-1 levels at all ages measured. As the liver is the major source of serum IGF-1, we examined differential expression in the livers of fasted female B6 and 6T mice by microarray.
Keywords: Genetic variation
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| Overall design |
The experimental design of this experiment was a simple two-factor experiment, with three biological replicates of each factor (in this case mouse strain, B6.C3H-6T vs. C57BL/6J). Each sample represented one mouse; no samples were pooled. No technical replicated were done.
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| Contributor(s) |
Ackert-Bicknell CL, Churchill GA, Affourtit J, Beamer WG, Rosen CJ |
| Citation(s) |
17584978 |
| Submission date |
Oct 03, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Cheryl Lynne Ackert-Bicknell |
| E-mail(s) |
cheryl.ackertb@jax.org
|
| Organization name |
The Jackson Laboratory
|
| Street address |
600 Main Street
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (6)
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| GSM138289 |
Liver_Chow_C57BL/6J_Female_8wks_Ped#36 |
| GSM138290 |
Liver_Chow_C57BL/6J_Female_8wks_Ped#37 |
| GSM138291 |
Liver_Chow_C57BL/6J_Female_8wks_Ped#38 |
| GSM138292 |
Liver_Chow_B6.C3H-6T_Female_8wks_Ped#39 |
| GSM138293 |
Liver_Chow_B6.C3H-6T_Female_8wks_Ped#40 |
| GSM138294 |
Liver_Chow_B6.C3H-6T_Female_8wks_Ped#41 |
|
| Relations |
| BioProject |
PRJNA97423 |
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