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| Status |
Public on Mar 31, 2015 |
| Title |
Expression data from human monocyte-derived dendritic cells treated or not with interleukin 17A (IL-17A) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
IL-17A is a pro-inflammatory cytokine that promotes host defense against infections and contributes to the pathogenesis of chronic inflammatory diseases. Dendritic cells (DC) are antigen-presenting cells responsible for adaptive immune responses. Here, we report that IL-17A induces intense remodeling of lipid metabolism in human monocyte-derived DC, as revealed by microarrays analysis. In particular NR1H3/LXR-a and its target genes were significantly upregulated in response to IL-17A. IL-17A induced accumulation of Oil Red O-positive lipid droplets in DC leading to the generation of lipid-laden DC. A lipidomic study established that all the analyzed lipid species, i.e phospholipids, cholesterol, triglycerides, cholesteryl esters were elevated in IL-17A-treated DC. The increased expression of membrane lipid transporters in IL-17A-treated DC as well as their enhanced ability to uptake the fatty acid Bodipy-FL-C16 suggested that lipid uptake was the main mechanism responsible for lipid accumulation in response to IL-17A. IL-17A-induced lipid laden DC were able to stimulate allogeneic T cell proliferation in vitro as efficiently as untreated DC, indicating that IL-17A-treated DC are potently immunogenic. This study, encompassed in the field of immunometabolism, points out for the first time IL-17A as a modulator of lipid metabolism in DC and provides a rationale to delineate the importance of lipid-laden DC in IL-17A-related inflammatory diseases.
We used microarrays analysis to understand the impact of IL-17A on human monocyte-derived human dendritic cells. We found overexpression of many genes involved in lipid metabolism in IL-17A-treated dendritic cells compared to untreated dendritic cells. In particular NR1H3/LXR-a and its target genes were significantly upregulated in response to IL-17A. IL-17A induced accumulation of Oil Red O-positive lipid droplets in DC leading to the generation of lipid-laden DC. A lipidomic study established that all the analyzed lipid species, i.e phospholipids, cholesterol, triglycerides, cholesteryl esters were elevated in IL-17A-treated DC. The increased expression of membrane lipid transporters in IL-17A-treated DC as well as their enhanced ability to uptake the fatty acid Bodipy-FL-C16 suggested that lipid uptake was the main mechanism responsible for lipid accumulation in response to IL-17A. IL-17A-induced lipid laden DC were able to stimulate allogeneic T cell proliferation in vitro as efficiently as untreated DC, indicating that IL-17A-treated DC are potently immunogenic. This study, encompassed in the field of immunometabolism, points out for the first time IL-17A as a modulator of lipid metabolism in DC and provides a rationale to delineate the importance of lipid-laden DC in IL-17A-related inflammatory diseases.
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| Overall design |
RNA was extracted from untreated in vitro-generated DC at day 0 (DC, 4 biological replicates ) or DC cultured for 12 days with IL-17A, in the absence or presence of IFN-g (DC-17 and DC-G17, 5 biological replicates)
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| Contributor(s) |
Delprat C, Mahtouk K |
| Citation(s) |
25833686 |
| Submission date |
Dec 10, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Karene Mahtouk |
| Organization name |
CNRS UMR5239
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| Department |
LBMC
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| Lab |
Dendritic cells and immune plasticity
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| Street address |
165 Chemin du Grand Revoyet : BP12
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| City |
Oullins |
| ZIP/Postal code |
69921 |
| Country |
France |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (9)
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| GSM1283274 |
Untreated dendritic cells, biological rep 1 (DC1) |
| GSM1283275 |
Untreated dendritic cells, biological rep 2 (DC2) |
| GSM1283276 |
Untreated dendritic cells, biological rep 3 (DC3) |
| GSM1283277 |
Untreated dendritic cells, biological rep 4 (DC5) |
| GSM1283278 |
IL-17A-treated dendritic cells, biological rep 1 (DC6-17) |
| GSM1283279 |
IL-17A-treated dendritic cells, biological rep 2 (DC4-17) |
| GSM1283280 |
IL-17A-treated dendritic cells, biological rep 3 (DC3-G17) |
| GSM1283281 |
IL-17A-treated dendritic cells, biological rep 4 (DC4-G17) |
| GSM1283282 |
IL-17A-treated dendritic cells, biological rep 5 (DC7-G17) |
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| Relations |
| BioProject |
PRJNA231027 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53163_RAW.tar |
41.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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