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| Status |
Public on Dec 31, 2013 |
| Title |
miR-483 overexpression in human sarcoma cell line MHH-ES-1. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
miRNA abnormalities are increasingly relevent to cancer development, We used microarrays to detail the global programme of gene expression upon miR-483 overexpression in sarcoma cell line MHH-ES-1.
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| Overall design |
MHH-ES-1 cells were transfected with miR-483-5p, -3p or scrambled control mimics and then harvested 48 hours after to isolate total RNAs using Trizol reagent (Invitrogen). Total RNA was converted to cRNA probes using the BioArray High Yield Transcript Labeling Kit (ENZO diagnostics), and hybridized to Affymetrix GeneChip human HG-U133ver2+ 3’-mRNA expression microarray chips using protocol EukGEs2v4 on the GeneChip Fluidic Station.
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| Contributor(s) |
Liu M, Roth A, Yu M, Morris R, Bersani F, Rivera M, Lu J, Vasudevan S, Ramaswamy S, Maheswaran S, Diederichs S, Haber DA |
| Citation(s) |
24298054 |
| Submission date |
Sep 18, 2013 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Mingzhu Liu |
| E-mail(s) |
mzliu@partners.org
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| Organization name |
MGH cancer center
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| Street address |
149 13th street
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| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA219469 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE50980_RAW.tar |
29.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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