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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jul 11, 2013 |
| Title |
Expression data from osteoblastic lineage cells isolated from normal and leukemic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Multipotent stromal cells (MSC) and their osteoblastic lineage cell (OBC) derivatives are part of the bone marrow (BM) niche and contribute to hematopoietic stem cell (HSC) maintenance. During myeloproliferative neoplasm (MPN) development, MSCs are stimulated to overproduce functtionally altered OBCs, which accumulate in the BM cavity as myelofibrotic cells. These MPN-expanded OBCs, in turn, impair the maintenance of normal HSCs but not of leukemic stem cells.
We used microarrays to detail the global gene expression changes in OBCs during BCR/ABL-induced MPN development, and understand the molecular deregulations contributing to their functional changes.
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| Overall design |
OBCs were isolated from diseased Scl-tTA::TRE-BCR/ABL (BA) mice and age-match controls around 5-6 weeks post-doxycycline withdrawal. Five independent biological replicate corresponding to single mice were used for each population. RNA was extracted, amplified and hybridized on Affymetrix Gene ST 1.0 microarrays.
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| Contributor(s) |
Koen S, Eric PM, Emmanuelle P |
| Citation(s) |
23850243 |
| Submission date |
Jul 01, 2013 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Emmanuelle Passegué |
| Organization name |
University of California San Francisco
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| Department |
Medicine
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| Lab |
Passegue Lab
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| Street address |
35 Medical Center Way, RMB-1017 Pod B, box 0667
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| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM1178732 |
Ctrl OBCs, post doxycyclin withdrawal, rep1 |
| GSM1178733 |
Ctrl OBCs, post doxycyclin withdrawal, rep2 |
| GSM1178734 |
Ctrl OBCs, post doxycyclin withdrawal, rep3 |
| GSM1178735 |
Ctrl OBCs, post doxycyclin withdrawal, rep4 |
| GSM1178736 |
Ctrl OBCs, post doxycyclin withdrawal, rep5 |
| GSM1178737 |
BA OBCs, post doxycyclin withdrawal, rep1 |
| GSM1178738 |
BA OBCs, post doxycyclin withdrawal, rep2 |
| GSM1178739 |
BA OBCs, post doxycyclin withdrawal, rep3 |
| GSM1178740 |
BA OBCs, post doxycyclin withdrawal, rep4 |
| GSM1178741 |
BA OBCs, post doxycyclin withdrawal, rep5 |
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| Relations |
| BioProject |
PRJNA210141 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48438_RAW.tar |
39.1 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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