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| Status |
Public on Apr 18, 2006 |
| Title |
Microarray analysis of gene expression during early stages of mild and severe cardiac hypertrophy |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Familial hypertrophic cardiomyopathy (FHC) is a disease characterized by ventricular hypertrophy, fibrosis, and aberrant systolic and/or diastolic function. We previously developed two transgenic mouse models that carry FHC associated mutations in alpha-tropomyosin (TM): FHC alpha-TM175 mice show patchy areas of mild ventricular disorganization and limited hypertrophy; whereas FHC alpha-TM180 mice exhibit severe hypertrophy and fibrosis and die within 6 months. To obtain a better understanding of the molecular mechanisms associated with the early onset of cardiac hypertrophy, we conducted a detailed comparative analysis of gene expression in 2.5-month-old control and FHC alpha-TM175 and alpha-TM180 ventricular tissue. Results show that 754 genes (from a total of 22,600) were differentially expressed between the NTG and the FHC hearts. There are 178 differentially regulated genes between NTG and the FHC alpha-TM175 hearts, 388 genes are differentially expressed between NTG and FHC alpha-TM180 hearts, and 266 genes are differentially expressed between FHC alpha-TM175 and FHC alpha-TM180 hearts. Genes that exhibit the largest increase in expression belong to the "secreted/extracellular matrix" category, and those with the most significant decrease in expression are associated with "metabolic enzymes."
Keywords: Cardiac hypertrophy, Tropomyosin, Mutation, Transgenic mouse, Microarray
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| Overall design |
Samples used for hybridization consisted of pooled (P) and non-pooled (NP) RNA extracts from the three groups namely NTG, alpha-TM175 and alpha-TM180. Ten hybridization experiments were performed in which each genotypic group was represented by two or more non-pooled (NP) individual RNA extracts and one pooled (P) sample that resulted from combining 20 individual heart extracts
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| Contributor(s) |
Rajan S, Williams SS, Aronow BJ, Wieczorek DF |
| Citation(s) |
16882888 |
| Submission date |
Apr 17, 2006 |
| Last update date |
Jan 08, 2019 |
| Contact name |
David F Wieczorek |
| E-mail(s) |
David.Wieczorek@uc.edu
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| Phone |
513-558-0058
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| Fax |
513-558-1885
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| Organization name |
University of Cincinnati
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| Department |
Molecular Genetics, Biochemistry and Microbiology
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| Street address |
231 Albert Sabin Way
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| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45267-0524 |
| Country |
USA |
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| Platforms (1) |
| GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA96783 |
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