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| Status |
Public on Mar 27, 2014 |
| Title |
MiR-221 mediated gene expression in human PCa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MiR-221 overexpression leads to activation of apoptosis, growth arrest and reduced invasivness in PCa cells. Interaction of miR-221 with potential target genes was analyzed by a genome wide expression profiling.. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real Time RT-PCR assay (IRF1, IRF2 SOCS3, STAT1), and Western Blotting. In total, 282 genes were upregulated and 64 downregulated based on a more than 2-fold difference to untransfected PC-3 cells. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, tumorigenesis and inflammation. We confirmed dysregulation of IRF-2 SOCS3, STAT1,IRF9. These results indicate that miR-221 overexpression might lead to activation of the JAK/STAT pathway and downregulation of miR-221 might contribute to tumorigenesis in PCa cells.
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| Overall design |
pre-miR-221 transfected PC-3 cells vs unstimulated control cells - total samples analysed are 4.
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| Contributor(s) |
Spahn M, Krebs M, Kneitz S, Kneitz B |
| Citation(s) |
24607843, 31828111 |
| Submission date |
Mar 29, 2013 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
susanne.kneitz@uni-wuerzburg.de
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| Phone |
+49-931-31 86526
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| Organization name |
University of Wuerzburg
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| Department |
Physiologigcal Chemistry
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| Street address |
Biozentrum, Am Hubland
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| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA195428 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE45627_RAW.tar |
20.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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