|
| Status |
Public on Feb 09, 2006 |
| Title |
Gene expression in murine germ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
In mammals, the X and Y chromosomes are subject to meiotic sex chromosome inactivation (MSCI) during prophase I in the male germline, but their status thereafter is currently unclear. An abundance of X-linked spermatogenesis genes has spawned the view that the X must be active [1-8]. On the other hand, the idea that the imprinted paternal X of the early embryo may be pre-inactivated by MSCI suggests that silencing may persist longer [9-12]. To clarify this issue, we establish a comprehensive X-expression profile during mouse spermatogenesis. Here, we discover that the X and Y occupy a novel compartment in the post-meiotic spermatid and adopt a non-Rabl configuration. We demonstrate that this post-meiotic sex chromatin (PMSC) persists throughout spermiogenesis into mature sperm and exhibits epigenetic similarity to the XY body. In the spermatid, 87% of X-linked genes remain suppressed post-meiotically, while autosomes are largely active. We conclude that chromosome-wide X-silencing continues from meiosis to the end of spermiogenesis and discuss implications for proposed mechanisms of imprinted X-inactivation.
Keywords: Specific germ cells from murine testis
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| |
|
| Overall design |
Independent germ cell preps were used for array analysis. Duplicates were provided for each sample.
|
| |
|
| Citation(s) |
16581510 |
| Submission date |
Feb 07, 2006 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Lizhong Yang |
| E-mail(s) |
lzyang@wsu.edu
|
| Phone |
1-509-335-2240
|
| Fax |
1-509-335-9688
|
| URL |
http://www.wsu.edu/~griswold
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Lab |
Griswold Lab/Center for Reproductive Biology
|
| Street address |
531 Fulmer Hall/POB 644660
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164-4660 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
|
| Samples (8)
|
| GSM95928 |
Type A spermatogonia replicate 1 |
| GSM95929 |
Type A spermatogonia replicate 2 |
| GSM95930 |
Type B spermatogonia replicate 1 |
| GSM95947 |
Type B spermatogonia replicate 2 |
| GSM95948 |
Pachytene spermatocytes replicate 1 |
| GSM95949 |
Pachytene spermatocytes replicate 2 |
| GSM95950 |
Round spermatids replicate 1 |
| GSM95951 |
Round spermatids replicate 2 |
|
| Relations |
| BioProject |
PRJNA95043 |
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