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| Status |
Public on Feb 01, 2012 |
| Title |
Systems approach identifies HIPK2 as a critical regulator of kidney tubulointerstitial fibrosis |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We used an integrated computational/experimental systems biology approach to identify upstream protein kinases that regulate gene expression changes in kidneys of HIV-1 transgenic mice (Tg26), which have significant tubulo-interstitial fibrosis (TIF) and glomerulosclerosis (GS). We identified the homeo-domain interacting protein kinase 2 (HIPK2) as a key regulator of TIF and GS. HIPK2 was upregulated in kidneys of Tg26 and patients with various kidney diseases. HIV infection increased the protein level of HIPK2 by promoting oxidative stress, which inhibited Siah1-mediated proteasomal degradation of HIPK2.
The data contain two sets: kidney corticies from WT and Tg26 mice and HEK293 transfected with HIPK2, HIPK2-DN and wild type.
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| Overall design |
Gene expression comparison between kidney cortecies of Tg26 HIV mouse model and wild type. Gene expression comparison between 293 HEK cells with HIPK-DN, HIPK-KO and normal.
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| Contributor(s) |
He JC, Ma'ayan A |
| Citation(s) |
22406746 |
| Submission date |
Jan 20, 2012 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Avi Ma'ayan |
| E-mail(s) |
avi.maayan@mssm.edu
|
| Phone |
2126591739
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Pharmacology and Systems Therapeutics
|
| Lab |
Ma'ayan Lab
|
| Street address |
One Gustave L. Levy Place Box 1215
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10037 |
| Country |
USA |
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| Platforms (2) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA150661 |
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